Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles

Acta Chimica Slovenica

Ali Tuncay Ozyilmaz¹  Esiye Irem Bayram¹  Ragibe Hulya Akyürekoğlu¹  Gul Ozyilmaz¹ 

[1] The University of Mustafa Kemal, Faculty of Arts & Sciences, Department of Chemistry 31040 Hatay/ Turkey;

关键词: amperometric biosensor;    polypyrrole;    poly(o-anisidine);    zinc oxide nanoparticles;    glucose oxidase;   

DOI  :  10.17344/acsi.2019.5171

来源: DOAJ

【 摘 要 】

GOD was immobilized onto polypyrrole (PPy) or poly(o-anisidine) (POA) coated Pt electrode to construct glucose sensitive biosensor. Because polymer film properties and enzyme activity affect the current response, PPy and POA synthesis conditions and also enzyme immobilization parameters were optimized in detail. The optimal monomer concentrations were determined as 25 and 50 mM for PPy and POA, respectively, whereas scan rate was 50 mV/s for both polymer films. In case of immobilization procedure, the optimal Chitosan (Chi), glucose oxidase (GOD) and glutaraldehyde (GAL) concentrations were determined as 0.5%, 2 mg/ml and 0.05% for PPy and 0.5%, 4 mg/ml and 0.075% for POA, respectively. Zinc oxide nanoparticles (ZnONP) were co-immobilized with GOD enzyme and it was revealed that ZnONP modification enhanced the efficiencies of both electrodes in terms of current responses and stabilities. Nyquist diagrams showed that enzyme electrodes were sensitive to glucose molecule and ZnONP modification improved the sensor efficiency.

【 授权许可】

Unknown