LC-MS/MS METHOD FOR THE SIMULTANEOUS DETERMINATION OF CEFEPIME AND TAZOBACTAM IN DOG PLASMA

Drug Analytical Research

Rajesh P. Chavan¹  Vineet S. Zope¹  Kiran R. Patil¹  Sukanta K. Naik¹  Ravindra D. Yeole¹ 

[1] Wockhardt Research Centre;

关键词: cefepime;    tazobactam;    liquid chromatography;    mass spectrometry;    bioanalysis;   

DOI  :  https://doi.org/10.22456/2527-2616.84357

来源: DOAJ

【 摘 要 】

A high performance liquid chromatography (HPLC) method with tandem mass spectrometric detection (MS/MS) has been developed and validated for the simultaneous quantification of cefepime and tazobactam in dog plasma. The method was developed on amide column with isocratic elution. The developed method is simple and economic in terms of sample preparation. The method is specific, sensitive, accurate, precise and robust. The method was successfully applied for pre-clinical pharmaco-kinetic studies in dogs. The Tmax was found to be 0.5 hr, the mean Cmax and AUC(0-12) displayed dose proportionate response.

【 授权许可】

Unknown