Antitumor Effects of a Sesquiterpene Derivative from Marine Sponge in Human Breast Cancer Cells

Marine Drugs

Chia-Hsien Feng¹  Jing-Ru Weng²  Wei-Yu Lin³  Li-Yuan Bai⁴  Jing-Lan Hu⁴  Chang-Fang Chiu⁴  Chih-Wen Shu⁵  Jui-Hsin Su⁶ 

[1] Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan;Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan;Department of Pharmacy, Kinmen Hospital, Ministry of Health and Welfare, Kinmen 89142, Taiwan;Division of Hematology and Oncology, Department of Internal Medicine, China Medical University Hospital, Taichung 40447, Taiwan;Institute of Biopharmaceutical Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan;National Museum of Marine Biology and Aquarium, Pingtung 94450, Taiwan;

关键词: ilimaquinone;    sponge;    cell cycle arrest;    apoptosis;    autophagy;    breast cancer;   

DOI  :  10.3390/md19050244

来源: DOAJ

【 摘 要 】

In this study, the anti-proliferative effect of ilimaquinone, a sesquiterpene derivative from the marine sponge, in breast cancer cells was investigated. Ilimaquinone inhibited the proliferation of MCF-7 and MDA-MB-231 breast cancer cells with IC₅₀ values of 10.6 μM and 13.5 μM, respectively. Non-tumorigenic human breast epithelial cells were less sensitive to ilimaquinone than breast cancer cells. Flow cytometric and Western blot analysis showed that ilimaquinone induced S-phase arrest by modulating the expression of p-CDC-2 and p21. Ilimaquinone induces apoptosis, which is accompanied by multiple biological biomarkers, including the downregulation of Akt, ERK, and Bax, upregulation of p38, loss of mitochondrial membrane potential, increased reactive oxygen species generation, and induced autophagy. Collectively, these findings suggest that ilimaquinone causes cell cycle arrest as well as induces apoptosis and autophagy in breast cancer cells.

【 授权许可】

Unknown