Exosome Mimetics-Loaded Hydrogel Accelerates Wound Repair by Transferring Functional Mitochondrial Proteins

Frontiers in Bioengineering and Biotechnology

Yunpeng Zhao¹  Zhixiao Liu¹  Qishu Jin²  Antong Du³  Jie Zhu³  Neng Ding³  Lie Zhu³  Hua Jiang⁴  Yue Wang⁵  Ling Wang⁶ 

[1] Department of Histology and Embryology, College of Basic Medicine, Naval Medical University, Shanghai, China;Department of Histology and Embryology, Harbin Medical University, Harbin, China;Department of Plastic and Reconstructive Surgery, Second Affiliated Hospital of Naval Medical University, Shanghai, China;Department of Plastic and Reconstructive Surgery, Shanghai East Hospital, Tongji University, Shanghai, China;Shanghai Key Laboratory of Cell Engineering, Shanghai, China;Stem Cell and Regeneration Medicine Institute, Research Center of Translational Medicine, Naval Medical University, Shanghai, China;

关键词: exosome mimetics;    hydrogel;    wound healing;    oxidative phoshorylation;    exosome;   

DOI  :  10.3389/fbioe.2022.866505

来源: DOAJ

【 摘 要 】

Loading human umbilical mesenchymal stem cell (hUMSC) derived exosomes onto hydrogel scaffolds is a strategy for rapid wound healing. The clinical application of exosomes is hindered by low production, and exosome mimetics could be substituted for exosomes. Here, the therapeutic effects of exosome-loaded hydrogels and exosome mimetic-loaded hydrogels on wounds are evaluated. Our results revealed that exosome mimetic-loaded hydrogels promote wound healing more efficiently than exosome-loaded hydrogels. Exosome mimetics can promote the proliferation and migration of dermal fibroblasts (hDF-a) cells in vitro. To investigate how exosome mimetics play a role, proteomics analysis was applied, and the obtained results suggested that exosome mimetics significantly enrich mitochondrial-derived oxidative phosphorylation-related proteins in comparison to exosomes. Overall, our work envisages the emerging potential of exosome mimetics, which take the advantage of exosomes and can be promising candidates for exosomes. It also suggests that hUMSC-derived exosome mimetic-loaded hydrogels have remarkable prospects for clinical application.

【 授权许可】

Unknown