Evaluation of sample treatments in a safe and straightforward procedure for the detection of SARS-CoV-2 in saliva

International Journal of Infectious Diseases

José J. Cerón¹  Enrique Bernal²  Cristina Sánchez Resalt³  Camila P. Rubio³  Lorena Franco-Martínez³  Asta Tvarijonaviciute³  Irene Martinez-Morata⁴  Alberto Torres-Cantero⁴  María R. Vicente-Romero⁵  María J. Alcaraz⁵  Silvia Martínez-Subiela⁶ 

[1] Corresponding authors.;Department of Environmental Health Sciences Mailman School of Public Health, Columbia University, New York, USA;Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, Murcia, Spain;Preventive Medicine, Hospital Clínico Universitario Virgen de la Arrixaca, IMIB, Universidad de Murcia, Spain;Unit of Infectious Diseases, Hospital General Universitario Reina Sofía, Universidad De Murcia, Murcia, Spain;Unit of Microbiology, Hospital General Universitaio Reina Sofía, Universidad De Murcia, Murcia, Spain;

关键词: SARS-CoV-2;    COVID-19;    Saliva;    RT-LAMP;    Assay;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objectives: To evaluate four sample treatments in a safe and straightforward procedure to detect SARS-CoV-2 in saliva. Methods: Four sample treatments were evaluated in a 3-step procedure to detect SARS-CoV-2 in saliva: 1) heating at 95 °C for 5 min for sample inactivation; 2) sample treatment; 3) analysis by reverse-transcription loop-mediated isothermal amplification (LAMP). Saliva samples used were from infected individuals or were spiked with known quantities of viral particles. Results: Three treatments had a limit of detection (LOD) of 500.000 viral particles per ml of saliva and could be used to detect individuals with potential to transmit the disease. The treatment of phosphate buffer, dithiothreitol, ethylenediaminetetraacetic acid and proteinase K, with an additional 95 °C heating step, yielded a lower LOD of 95; its sensitivity ranged from 100% in patients with nasopharyngeal swab reverse-transcriptase polymerase chain reaction cycle threshold values <20 to 47.8% for values >30. Conclusions: This report highlights the importance of an adequate sample treatment for saliva to detect SARS-CoV-2 and describes a flexible procedure that can be adapted to point-of-care. Although its sensitivity when LAMP is used is lower than reverse-transcriptase polymerase chain reaction, this procedure can contribute to COVID-19 control by detecting individuals able to transmit the disease.

【 授权许可】

Unknown