Biological and Chemical Insights of Beech (Fagus sylvatica L.) Bark: A Source of Bioactive Compounds with Functional Properties

Antioxidants

Daniela-Lucia Muntean¹  DanC. Vodnar²  Alexandru Gavan³  Ovidiu Crișan⁴  Sanda Coșarcă⁵  Corneliu Tanase⁵  Ana-Maria Gheldiu⁶  Andrei Mocan⁶  Alexandru Nicolescu⁶ 

[1] Department of Analytical chemistry and Drug analysis, University of Medicine, Pharmacy, Sciences and Technology “, 540139 Târgu Mureș, Romania;Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania;Department of Medical Devices, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca 400439, Romania;Department of Organic Chemistry, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania;Department of Pharmaceutical Botany, University of Medicine, Pharmacy, Sciences and Technology, 540139 Târgu Mureș, Romania;Department of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca 400337, Romania;

关键词: antioxidant;    antibacterial;    antifungal;    antimutagen;    anti-α-glucosidase;    anti-tyrosinase;    beech bark;    microwave assisted extraction;    optimization;   

DOI  :  10.3390/antiox8090417

来源: DOAJ

【 摘 要 】

The present study aimed, on the one hand, to improve the yield of microwave assisted extraction (MAE) of polyphenols from beech bark by using a design of experiments (DoE) approach. On the other hand, beech bark extracts (BBE) were characterized in terms of their phytochemical profile and evaluated for biological potential (antioxidant, antibacterial, antifungal, antimutagen, anti-α-glucosidase, and anti-tyrosinase). The extraction time varies with the amount of extracted total phenolic content (TPC). The microwave power favors TPC extraction but in different proportions. The optimum conditions which gave the highest TPC (76.57 mg GAE/g dry plant material) were reached when the microwave power was 300 W, extraction time was 4 min, and the solvent was an ethanol−water (50:50) mixture. The practical value of TPC after a controlled experiment was 76.49 mg GAE/g plant material. The identified compounds were vanillic acid, gallic acid, epicatechin, catechin, protocatechuic acid, chlorogenic acid, ferulic acid, and isoquercitrin. The antioxidant potential of BBEs was demonstrated by in vitro experiments. The BBEs were active against Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhimurium, Escherichia coli, and Candida species. All extracts were antimutagenic and expressed an inhibition on α-glucosidase and tyrosinase activity. Regarding antimutagen activity, the assayed extracts may be considered to have low or no antimutagen effects.

【 授权许可】

Unknown