Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman Primates

Biology

Paul L. A. M. Corstjens¹  Elisa M. Tjon Kon Fat¹  Zijie Zhou²  Annemieke Geluk²  Anouk van Hooij²  Tom H. M. Ottenhoff²  Richard Vervenne³  Claudia C. Sombroek³  Frank Verreck³ 

[1] Department of Cell and Chemical Biology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands;Department of Infectious Diseases, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands;Section of TB Research & Immunology, Biomedical Primate Research Center (BPRC), 2288 GJ Rijswijk, The Netherlands;

关键词: biomarkers;    diagnosis;    nonhuman primates (NHPs);    lateral flow assay;    up-converting reporter particles;    MTB;   

DOI  :  10.3390/biology10121260

来源: DOAJ

【 摘 要 】

Nonhuman primates (NHPs) are relevant models to study the pathogenesis of tuberculosis (TB) and evaluate the potential of TB therapies, but rapid tools allowing diagnosis of active pulmonary TB in NHPs are lacking. This study investigates whether low complexity lateral flow assays utilizing upconverting reporter particles (UCP-LFAs) developed for rapid detection of human serum proteins can be applied to detect and monitor active pulmonary TB in NHPs. UCP-LFAs were used to assess serum proteins levels and changes in relation to the MTB challenge dosage, lung pathology, treatment, and disease outcome in experimentally MTB-infected macaques. Serum levels of SAA1, IP-10, and IL-6 showed a significant increase after MTB infection in rhesus macaques and correlated with disease severity as determined by pathology scoring. Moreover, these biomarkers could sensitively detect the reduction of bacterial levels in the lungs of macaques due to BCG vaccination or drug treatment. Quantitative measurements by rapid UCP-LFAs specific for SAA1, IP-10, and IL-6 in serum can be utilized to detect active progressive pulmonary TB in macaques. The UCP-LFAs thus offer a low-cost, convenient, and minimally invasive diagnostic tool that can be applied in studies on TB vaccine and drug development involving macaques.

【 授权许可】

Unknown