BODIPY-based dye for no-wash live-cell staining and imaging

BioTechniques

Stepan P. Chumakov¹  Natalia M. Ratnikova¹  Alexey A. Pakhomov¹  Igor E. Deyev¹  Vladimir I. Martynov¹  Veronika B. Mironiuk¹  Aziz M. Muzafarov²  Yuriy N. Kononevich² 

[1] 1M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation;2A.N. Nesmeyanov Institute of Organoelement Compounds, Moscow, Russian Federation;

关键词: fluorophore;    aggregation-caused quenching;    BODIPY;    live-cell imaging;    flow cytometry;    fluorescence microscopy;   

DOI  :  10.2144/000114577

来源: DOAJ

【 摘 要 】

In nonpolar solvents, hydrophobic organic fluorophores often show bright fluorescence, whereas in polar media, they usually suffer from aggregation-caused quenching (ACQ). Here, we harnessed this solvatochromic behavior of a 1,3,5,7-tetramethyl-BODIPY derivative for cell staining and applied it to live-cell imaging and flow cytometry. As opposed to commercially available dyes, this BODIPY derivative showed excellent contrast immediately after staining and did not require any wash-off.

【 授权许可】

Unknown