Spectroscopic and In Vitro Investigations of Boron(III) Complex with Meso-4-Methoxycarbonylpropylsubstituted Dipyrromethene for Fluorescence Bioimaging Applications

Molecules

卷:25

Galina Guseva¹  Elena Antina¹  Mikhail Berezin¹  Airat Kayumov²  Olga Lodochnikova²  Daut Islamov²  Konstantin Usachev²  Roman Pavelyev²  Sergei Boichuk³  Liliya Nikitina³  Svetlana Lisovskaya³ 

[1] G.A. Krestov Institute of Solution Chemistry of Russian Academy of Sciences, 1 Akademicheskaya st., 153045 Ivanovo, Russia;

[2] Kazan Federal University, 18 Kremlyovskaya st., 420008 Kazan, Russia;

[3] Kazan State Medical University, 49 Butlerova st., 420012 Kazan, Russia;

关键词: dipyrromethene;    BODIPY;    fluorophore;    biomarker;    pathogenic microorganisms;   

DOI  :  10.3390/molecules25194541

来源: DOAJ

【 摘 要 】

This study focuses on the behavior of a new fluorescent marker for labeling individual biomolecules and staining cell organelles developed on a meso-substituted BODIPY platform. Boron(III) complex with meso-4-methoxycarbonylpropylsubstituted 3,3’,5,5’-tetramethyl-2,2′-dipyrromethene has been synthesized and identified via visible, UV-, NMR- and MS-spectra X-ray. The behavior of fluorophore in solutions has been studied with various experimental techniques. It has been found that luminophore exhibits a high quantum yield (almost ~100–75%) in the blue-green region (513–520 nm) and has high photostability. In addition, biological analysis indicates that the fluorophore exhibits a tendency to effectively penetrate into cell membranes. On the other hand, the proposed BODIPY can be used to study the significant differences among a large number of pathogens of mycotic infections, as well as to visualize structural changes in the plasma membrane, which is necessary for the clearance of mammalian cells undergoing apoptotic cell death.

【 授权许可】

Unknown