Solvent Mixture Optimization in the Extraction of Bioactive Compounds and Antioxidant Activities from Garlic (Allium sativum L.)

Molecules

Altino Júnior Mendes Oliveira¹  Maysa Mathias Alves Pereira¹  Laís Campelo Mendes¹  João Pedro Miranda Rocha¹  Adriane Duarte Coelho¹  Moacir Pasqual¹  Vytória Piscitelli Cavalcanti¹  Suzan Kelly Vilela Bertolucci¹  Ludmila Caproni Morais¹  Joyce Dória¹  Moacir Rossi Forim²  Smail Aazza³ 

[1] Department of Agriculture, Federal University of Lavras (UFLA), Lavras 37200-900, Brazil;Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos 13565-905, Brazil;Laboratory of Phytochemistry, National Agency of Medicinal and Aromatic Plants (NAMAP/ANPMA), Taounate 34202, Morocco;

关键词: extraction optimization;    solvent mixtures;    response surface methodology;    phenols;    thiosulfinates;    antioxidants;   

DOI  :  10.3390/molecules26196026

来源: DOAJ

【 摘 要 】

Garlic is a health promoter that has important bioactive compounds. The bioactive extraction is an important step in the analysis of constituents present in plant preparations. The purpose of this study is to optimize the extraction with the best proportion of solvents to obtain total phenolic compounds (TPC) and thiosulfinates (TS) from dried garlic powder, and evaluate the antioxidant activities of the optimized extracts. A statistical mixture simplex axial design was used to evaluate the effect of solvents (water, ethanol, and acetone), as well as mixtures of these solvents, after two ultrasound extraction cycles of 15 min. Results showed that solvent mixtures with a high portion of water and pure water were efficient for TPC and TS recovery through this extraction procedure. According to the regression model computed, the most significant solvent mixtures to obtain high TPC and TS recovery from dried garlic powder are, respectively, the binary mixture with 75% water and 25% acetone and pure water. These optimized extracts presented oxygen radical absorbance capacity. Pure water was better for total antioxidant capacity, and the binary mixture of water–acetone (75:25) was better for DPPH scavenging activity. These optimized extracts can be used for industrial and research applications.

【 授权许可】

Unknown