In-Parallel Polar Monitoring of Chemiluminescence Emission Anisotropy at the Solid–Liquid Interface by an Optical Fiber Radial Array

Chemosensors

Mara Mirasoli¹  Elisa Marchegiani¹  Aldo Roda¹  Massimo Guardigli¹  Martina Zangheri¹  Francesco Baldini²  Ambra Giannetti²  Simone Berneschi²  Sara Tombelli²  Cosimo Trono² 

[1] Department of Chemistry “Giacomo Ciamician”, Alma Mater Studiorum—University of Bologna, via Selmi 2, 40126 Bologna (BO), Italy;Institute of Applied Physics “Nello Carrara”, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy;

关键词: chemiluminescence;    emission anisotropy;    multimode optical fiber;    ccd camera detector;   

DOI  :  10.3390/chemosensors8010018

来源: DOAJ

【 摘 要 】

Chemiluminescence (CL) detection is widely employed in biosensors and miniaturized analytical devices since it offers high detectability and flexible device design (there are no geometry requirements for the measurement cell, except the ability to collect the largest fraction of emitted photons). Although the emission anisotropy phenomenon for an emitting dipole bound to the interface between two media with different refractive index is well known for fluorescence, it is still poorly investigated for CL reactions, in which the excited-state reaction products can diffuse in solution before the photon emission event. In this paper, we propose a simple method for the real-time evaluation of the CL emission anisotropy based on a radial array of optical fibers, embedded in a poly(methyl methacrylate) semicylinder and coupled with a Charge-Coupled Device (CCD) camera through a suitable interface. The polar-time evolutions of the CL emission have been studied for catalyzing enzymes immobilized onto a solid surface (heterogeneous configuration) or free in solution (homogeneous configuration). Evidence of the anisotropy phenomenon is observed, indicating that the lifetime of the excited-state products of the enzyme-catalyzed reactions is shorter than the time required for their diffusion in solution at a distance at which the CL can be considered isotropic. These results open new perspectives in the development of CL-based miniaturized analytical devices.

【 授权许可】

Unknown