Application of β-cyclodextrin/MnFe2O4 magnetic nanoparticles as a catalyst for fast chemiluminescence determination of glutathione in human blood using luminol-diperiodatoargentate(III) System

Journal of the Brazilian Chemical Society

Isfahan University of Technology, Isfahan, I. R. Iran¹  Haghighatnia, Fariba¹  Rezaei, Behzad¹  Ensafi, Ali A.¹  Aalaye, Saeed E.¹ 

关键词: chemiluminescence;    MnFe2O4 magnetic nanoparticles;    glutathione;    human blood;    luminol-diperiodatoargentate(III);   

DOI  :  10.1590/S0103-50532012001200014

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

B> This work is based on the enhanced chemiluminescence (CL) of the reaction between luminol and diperiodatoargentate(III) (DPA) {K[Ag(H3IO6)2]} in alkaline solution for determination of glutathione (GSH). In the presence of inclusion complex β-cyclodextrin (β-CD)/MnFe2O4 magnetic nanoparticles (MNPs), the experimental results showed that the CL emission was very fast (response time of about 1 s, which is a very important parameter in the clinical analysis) and sharp (CL signal increased 9 times). The catalytic effects of β-CD/MnFe2O4 MNPs on the CL response of luminol-DPA system were investigated. The linearly of method depended on GSH concentration in the ranges of 5.0 × 10–8 to 4.0 × 10–6 mol L–1 and limit of detection calculated was 1.5 × 10–8 mol L–1 and relative standard deviation of 2.8% for 9 replicated measurements of 8.0 × 10–7 mol L–1 GSH. The proposed method was successfully applied for rapid and sensitive determination of GSH in human blood samples.

【 授权许可】

Unknown