Simulated Flock-Level Shedding Characteristics of Turkeys in Ten Thousand Bird Houses Infected with H7 Low Pathogenicity Avian Influenza Virus Strains

Viruses

Amos Ssematimba¹  Kaitlyn M. St. Charles¹  Carol J. Cardona¹  Emily Walz¹  David A. Halvorson¹  Peter J. Bonney¹  Sasidhar Malladi¹  Marie R. Culhane¹ 

[1] Secure Food Systems Team, Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA;

关键词: avian influenza;    epidemiology;    mathematical modeling;    outbreak management;    poultry;   

DOI  :  10.3390/v13122509

来源: DOAJ

【 摘 要 】

Understanding the amount of virus shed at the flock level by birds infected with low pathogenicity avian influenza virus (LPAIV) over time can help inform the type and timing of activities performed in response to a confirmed LPAIV-positive premises. To this end, we developed a mathematical model which allows us to estimate viral shedding by 10,000 turkey toms raised in commercial turkey production in the United States, and infected by H7 LPAIV strains. We simulated the amount of virus shed orally and from the cloaca over time, as well as the amount of virus in manure. In addition, we simulated the threshold cycle value (Ct) of pooled oropharyngeal swabs from birds in the infected flock tested by real-time reverse transcription polymerase chain reaction. The simulation model predicted that little to no shedding would occur once the highest threshold of seroconversion was reached. Substantial amounts of virus in manure (median $1.5\times {10}^8$ and $5.8\times {10}^9$; 50% egg infectious dose) were predicted at the peak. Lastly, the model results suggested that higher Ct values, indicating less viral shedding, are more likely to be observed later in the infection process as the flock approaches recovery.

【 授权许可】

Unknown