Patterns of microRNAs 142-3p, 106a, 17 and 20a expression in patients with systemic lupus erythematosus

Egyptian Rheumatologist

Asmaa Ali¹  Nahed Emara²  Eman Awadallah²  Rasha N. Yousef²  Hecham Gamal AlDeen²  Soha A. Nasr²  Abeer Ramadan³  Alshaimaa R. Alnaggar⁴  Mervat E. Behiry⁴  Noha M. Khalil⁴ 

[1] Chest Department, Abbassia Chest Hospital, Ministry of Health and Population, Cairo, Egypt;Department of Clinical and Chemical Pathology, National Research Centre, Giza, Egypt;Department of Molecular Genetics and Enzymology, National Research Centre, Giza, Egypt;Internal Medicine Department, Rheumatology and Clinical Immunology Unit, KasrAlainy School of Medicine, Cairo University, Egypt;

关键词: SLE;    miR-142-3p;    miR-106a;    miR-20a;    miR-17;    SLEDAI;   

DOI  :  

来源: DOAJ

【 摘 要 】

Introduction: Systemic lupus erythematosus (SLE) is a connective tissue disorder which involves immune system dysregulation. Micro-ribonucleic acids (MiRNAs) up and down regulation are implicated in its development. Aim of the work: To examine the expression levels of certain miRNAs (miR-17, miR-20a, miR-106a, and miR-142-3p) in SLE patients and to investigate which miRNAs are involved in the pathogenesis of SLE, in order to be used as diagnostic or prognostic biomarkers. Patients and methods: 60 patients and 60 matched control were included. SLE disease activity index 2000 (SLEDAI-2 K) was assessed. Assessment of serum miRNAs was done using real-time quantitative polymerized chain reaction. Results: The median age of patients was 29.5 years (24–32) and they were 59 females and 1 male with a median disease duration of 24 (20–48) months and SLEDAI of 5.5 (2.3–9). miR-17a, miR-142, miR-20a and miR-106a were significantly lower in patients (22.5 ± 2.2; 22.8 ± 2.2; 23.4 ± 2.4 and 22.6 ± 2.2) compared with control (23.8 ± 2.1; 24.7 ± 2.2; 25.1 ± 2.4 and 24.4 ± 2.3) (p = 0.002, p < 0.001, p < 0.001 and p < 0.001 respectively). The 4 markers significantly correlated with the SLEDAI (p = 0.002; p = 0.002; p = 0.004 and p = 0.001 respectively). The diagnostic capability of miR-142, miR-20a and miR-106a in predicting SLE showed a specificity of 95%, 98% and 90% at cut-off values of >22.6, >22.1 and >22.8 respectively; area under the curve was 67, 72, 70 and 76% at p-values p = 0.19, p < 0.001, p < 0.001, p < 0.001 respectively. Conclusion: MiR-17, miR-142-3p, miR-20a, and miR-106a have a diagnostic value in SLE and may serve as a therapeutic target for treatment. The studied markers were also related to the disease activity.

【 授权许可】

Unknown