Involvement of miR-20a in Promoting Gastric Cancer Progression by Targeting Early Growth Response 2 (EGR2)

International Journal of Molecular Sciences

Xiangsheng Li²  Zhichao Zhang²  Ming Yu²  Liqi Li²  Guangsheng Du²  Weidong Xiao¹ 

[1] Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Xinqiao Road, Chongqing 400037, China;

关键词: gastric cancer;    miR-20a;    early growth response 2;    carcinogenesis;    chemoresistance;   

DOI  :  10.3390/ijms140816226

来源: mdpi

【 摘 要 】

Gastric cancer (GC) is one of the most common cancers, with high incidences in East Asia. microRNAs (miRNAs) play essential roles in the carcinogenesis of GC. miR-20a was elevated in GC, while the potential function of miR-20a was poorly understood. miR-20a expression was examined in GC tissues and cell lines. The effects of miR-20a on the growth, migration, invasion, and chemoresistance of GC cells were examined. Luciferase reporter assay and Western blot were used to screen the target of miR-20a. miR-20a was increased in GC tissues and cell lines. miR-20a promoted the growth, migration and invasion of GC cells, enhanced the chemoresistance of GC cells to cisplatin and docetaxel. Luciferase activity and Western blot confirmed that miR-20a negatively regulated EGR2 expression. Overexpression of EGR2 significantly attenuated the oncogenic effect of miR-20a. miR-20a was involved in the carcinogenesis of GC through modulation of the EGR2 signaling pathway.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland