Alteration in expression level of some growth and stress-related genes after rhizobacteria inoculation to alleviate drought tolerance in sensitive rice genotype

Chemical and Biological Technologies in Agriculture

Haytham M. Abd-Elhalim¹  Jacek Wrobel²  Hazem M. Kalaji³  Mohamed H. Abdelfattah⁴  Medhat E. Eldenary⁴  Samar A. Omar⁴  Nashwa A. H. Fetyan⁵ 

[1] Agricultural Genetics Engineering Research Institute, Agriculture Research Center;Department of Bioengineering, West Pomeranian University of Technology in Szczecin;Department of Plant Physiology, Institute of Biology, Warsaw-University of Life Science SGGW;Genetic Department, Faculty of Agriculture, Tanta University;Soils, Water and Environmental Research Institute, Agriculture Research Center;

关键词: Rice;    Drought stress;    PGPRs inoculation;    Rhizobacteria enzyme activities;    MDA;    Chlorophyll pigments;   

DOI  :  10.1186/s40538-021-00237-4

来源: DOAJ

【 摘 要 】

Abstract Background Drought stress is one of the major abiotic stresses that adversely affect rice production. Four rice genotypes, Giza177, IR64 (as sensitive genotypes) and Vandana, Orabi3 (as tolerant genotypes) were used to screen and characterize the soil microbes associated with each genotype under drought stress. Results The soil microbes associated with the tolerant genotypes showed high drought tolerance and high levels of enzyme activity. The most drought-tolerant isolates were inoculated with the sensitive genotype Giza177 under drought conditions. Some morphological, biochemical and molecular responses of inoculated plants were estimated. Inoculated plants showed regulation of some growth and stress-related genes (COX1, AP2-EREBP, GRAM, NRAMP6, NAM, GST, DHN and three genes of expansin (EXP1, EXP2 and EXP3) under drought conditions. Expression profiling of these genes were highly induced in plants inoculated with 4E11 and were correlated with improved growth status under drought stress. Conclusion Based on this, drought-tolerant plant growth-promoting rhizobacteria (PGPRs) were associated with the drought-tolerant genotype (Orabi 3). They were related to the significant increase in soil enzymes activities (dehydrogenase, nitrogenase, urease and alkaline phosphatase) in the rhizosphere of tolerant genotype. Inoculation the drought-sensitive genotype (Giza 177) with the most drought-tolerant isolates improved the tolerance status of the sensitive rice genotype and induced the expression of some growth and stress-responsive genes. AP2-EREBP, NRAMP6, DHN and all expansin genes (EXP1, EXP2 and EXP3) were the highly induced genes in inoculated plants with 4E11 strain and the consortium of three selected strains under drought condition. Graphic abstract

【 授权许可】

Unknown