Ultrasound Protects Human Chondrocytes from Biochemical and Ultrastructural Changes Induced by Oxidative Stress

Applied Sciences

Rodica Ana Ungur¹  Viorela Mihaela Ciortea¹  Laszlo Irsay¹  Ileana Monica Borda¹  Ștefana Bâlici²  Eleonora Dronca²  Adrian Florea²  Simona Căinap³  Adriana Muresan⁴  Şoimiţa Mihaela Suciu⁴  Diana Elena Olteanu⁴  Ioana Anamaria Onac⁵  Răzvan Andrei Codea⁶  Ciprian Andrei Ober⁶  Georgiana Smaranda Martiș (Petruț)⁷ 

[1] Department of Medical Specialties, Faculty of Medicine, Iuliu-Hațieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, Romania;Department of Molecular Sciences, Faculty of Medicine, Iuliu-Hațieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, Romania;Department of Mother and Child, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, Romania;Department of Physiology, Faculty of Medicine, Iuliu-Hațieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, Romania;Department of Rheumatology, Surrey and Sussex Health Care NHS Trust, Canada Ave, Redhill RH1 5RH, UK;Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, 3-5 Mănăștur Street, 400372 Cluj-Napoca, Romania;Food Engineering Department, University of Agricultural Sciences and Veterinary Medicine, 64 Calea Floresti, 400509 Cluj-Napoca, Romania;

关键词: ultrasound;    chondrocytes;    assessment;    oxidative stress;    TNF-α;    autophagosomes;   

DOI  :  10.3390/app12052334

来源: DOAJ

【 摘 要 】

The aim of the study was to assess the effects of therapeutic ultrasound (US) on oxidative stress (OS)-induced changes in cultured human chondrocytes (HCH). For this, monolayer HCH were randomized in three groups: a control group (CG), a group exposed to OS (OS group), and a group exposed to US and OS (US-OS group). US exposure of the chondrocytes was performed prior to OS induction by hydrogen peroxide. Transmission electron microscopy (TEM) was used to assess the chondrocytes ultrastructure. OS and inflammatory markers were recorded. Malondialdehyde (MDA) and tumor necrosis factor (TNF)-α were significantly higher (p < 0.05) in the OS group than in CG. In the US-OS group MDA and TNF-α were significantly lower (p < 0.05) than in the OS group. Finally, in the US-OS group MDA and TNF-α were lower than in CG, but without statistical significance. TEM showed normal chondrocytes in CG. In the OS group TEM showed necrotic chondrocytes and chondrocytes with a high degree of vacuolation and cell organelles damages. In the US-OS group the chondrocytes ultrastructure was well preserved, and autophagosomes were generated. In conclusion, US could protect chondrocytes from biochemical (lipid peroxidation, inflammatory markers synthesis) and ultrastructural changes induced by OS and could stimulate autophagosomes development.

【 授权许可】

Unknown