Non-permissive SARS-CoV-2 infection in human neurospheres

Stem Cell Research

Rafael Dariolli¹  Ismael C. Gomes²  Diogo Biagi²  Leticia R.Q. Souza³  Suelen da Silva Gomes Dias⁴  Teresa Puig-Pijuan⁴  Luiz G.H.S. Aragão⁵  Gabriela Vitória⁶  Mayara A. Mendes⁶  Helena L. Borges⁶  Daniel R. Furtado⁶  Patrícia T. Bozza⁶  Carla Veríssimo⁶  Estela M. Cruvinel⁶  Carolina da S.G. Pedrosa⁶  Karina Karmirian⁶  Carolina Q. Sacramento⁶  Livia Goto-Silva⁶  Isis M. Ornelas⁶  Julia T. Oliveira⁷  Vinicius Cardoso Soares⁷  Thayana C.Q. Torquato⁷  José A. Salerno⁷  Natalia Fintelman-Rodrigues⁸  Stevens Rehen⁸  Jairo R. Temerozo⁹  Marília Zaluar P. Guimarães^1,10  Thiago Moreno L. Souza^1,10 

[1] Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil;Institute of Biomedical Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil;Laboratory on Thymus Research, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil;National Institute for Science and Technology on Innovation in Diseases of Neglected Populations (INCT/IDPN), Center for Technological Development in Health (CDTS), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil;Program of Immunology and Inflammation, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil;D’Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, Brazil;Immunopharmacology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil;Institute of Biomedical Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil;National Institute for Science and Technology on Neuroimmunomodulation (INCT/NIM), Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil;Pluricell Biotech, São Paulo, SP, Brazil;

关键词: New coronavirus;    Brain;    Neurospheres;    SARS-CoV-2;    iPS;   

DOI  :  

来源: DOAJ

【 摘 要 】

Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that several other organs are affected, including the brain. Neurological manifestations such as stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but the neurotropic potential of the virus is still debated. Herein, we sought to investigate SARS-CoV-2 infection in human neural cells. We demonstrated that SARS-CoV-2 infection of neural tissue is non-permissive, however, it can elicit inflammatory response and cell damage. These findings add to the hypothesis that most of the neural damage caused by SARS-CoV-2 infection is due to a systemic inflammation leading to indirect harmful effects on the central nervous system despite the absence of local viral replication.

【 授权许可】

Unknown