Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes

BMC Genomics

Bruno Dallagiovanna¹  Isabela Tiemy Pereira¹  Anny Waloski Robert¹  Marco Augusto Stimamiglio¹  Hugo Naya²  Rocío Amorín²  Lucia Spangenberg² 

[1] Basic Stem-cell Biology Laboratory, Instituto Carlos Chagas - FIOCRUZ-PR;Bioinformatics Unit, Institut Pasteur de Montevideo;

关键词: Cardiomyogenesis;    hESC-derived cardiomyocytes;    Polysome profiling;    Post-transcriptional regulation;   

DOI  :  10.1186/s12864-019-5550-3

来源: DOAJ

【 摘 要 】

Abstract Background Cardiac cell fate specification occurs through progressive steps, and its gene expression regulation features are still being defined. There has been an increasing interest in understanding the coordination between transcription and post-transcriptional regulation during the differentiation processes. Here, we took advantage of the polysome profiling technique to isolate and high-throughput sequence ribosome-free and polysome-bound RNAs during cardiomyogenesis. Results We showed that polysome-bound RNAs exhibit the cardiomyogenic commitment gene expression and that mesoderm-to-cardiac progenitor stages are strongly regulated. Additionally, we compared ribosome-free and polysome-bound RNAs and found that the post-transcriptional regulation vastly contributes to cardiac phenotype determination, including RNA recruitment to and dissociation from ribosomes. Moreover, we found that protein synthesis is decreased in cardiomyocytes compared to human embryonic stem-cells (hESCs), possibly due to the down-regulation of translation-related genes. Conclusions Our data provided a powerful tool to investigate genes potentially controlled by post-transcriptional mechanisms during the cardiac differentiation of hESC. This work could prospect fundamental tools to develop new therapy and research approaches.

【 授权许可】

Unknown