BMP signaling regulates Nkx2‐5 activity during cardiomyogenesis

FEBS Letters

Rogerson, Parker J¹  Jamali, Mina¹  Skerjanc, Ilona S¹  Karamboulas, Christina¹ 

[1] Department of Biochemistry, Medical Sciences Building, The University of Western Ontario, London, ON, Canada N6A 5C1

关键词: Cardiomyogenesis;    P19 cell;    Embryonal carcinoma;    Nkx2-5;    Bone morphogenetic protein;    BMPs;    bone morphogenetic proteins;    MyHC;    myosin heavy chain;    PGK;    phosphoglycerate kinase;   

DOI  :  10.1016/S0014-5793(01)03151-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nkx2-5 regulates the transcription of muscle-specific genes during cardiomyogenesis. Nkx2-5 expression can induce cardiomyogenesis in aggregated P19 cells but not in monolayer cultures. In order to investigate the mechanism by which cellular aggregation regulates Nkx2-5 function, we examined the role of bone morphogenetic protein 4 (BMP4). We showed that the expression of the BMP inhibitor, noggin, was sufficient to inhibit the induction of cardiomyogenesis by Nkx2-5 during cellular aggregation. Furthermore, soluble BMP4 could activate Nkx2-5 function in monolayer cultures, resulting in the formation of cardiomyocytes. Therefore, BMP signaling is necessary and sufficient for the regulation of Nkx2-5 activity during cardiomyogenesis in P19 cells.

【 授权许可】

Unknown