Intake of flavonoids from Astragalus membranaceus ameliorated brain impairment in diabetic mice via modulating brain-gut axis

Chinese Medicine

Zhilong Liu¹  Dingkun Gui²  Weiming Wang³  Wenbo Huang³  Xuling Li³  Tingting Zhao³  Junling Gu³  Zhe Wang³  Kin-Fong Hong³  Youhua Xu³  Hua Zhou³  Jing Lin⁴  Zhenghai Li⁵  Rushang Wang⁵  Tingting Duan⁵  Ruixue Dong⁶ 

[1] Department of Endocrinology, Zhuhai Hospital of Integrated Traditional Chinese and Western Medicine;Department of Nephrology, Shanghai Jiao Tong University Affiliated to Sixth People’s Hospital;Faculty of Chinese Medicine, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology;Guangdong Provincial Hospital of Chinese Medicine-Zhuhai Hospital;Institute of Consun Co. for Chinese Medicine in Kidney Diseases, Guangdong Consun Pharmaceutical Group;School of Pharmacy, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology;

关键词: Astragalus membranaceus;    Brain;    Diabetes;    Gut;    Microbiota;   

DOI  :  10.1186/s13020-022-00578-8

来源: DOAJ

【 摘 要 】

Abstract Background Brain impairment is one of a major complication of diabetes. Dietary flavonoids have been recommended to prevent brain damage. Astragalus membranaceus is a herbal medicine commonly used to relieve the complications of diabetes. Flavonoids is one of the major ingredients of Astragalus membranaceus, but its function and mechanism on diabetic encepholopathy is still unknown. Methods Type 2 diabetes mellitus (T2DM) model was induced by high fat diet and STZ in C57BL/6J mice, and BEnd.3 and HT22 cell lines were applied in the in vitro study. Quality of flavonoids was evaluated by LC–MS/MS. Differential expressed proteins in the hippocampus were evaluated by proteomics; influence of the flavonoids on composition of gut microbiota was analyzed by metagenomics. Mechanism of the flavonoids on diabetic encepholopathy was analyzed by Q-PCR, Western Blot, and multi-immunological methods et al. Results We found that flavonoids from Astragalus membranaceus (TFA) significantly ameliorated brain damage by modulating gut-microbiota-brain axis: TFA oral administration decreased fasting blood glucose and food intake, repaired blood brain barrier, protected hippocampus synaptic function; improved hippocampus mitochondrial biosynthesis and energy metabolism; and enriched the intestinal microbiome in high fat diet/STZ-induced diabetic mice. In the in vitro study, we found TFA increased viability of HT22 cells and preserved gut barrier integrity in CaCO2 monocellular layer, and PGC1α/AMPK pathway participated in this process. Conclusion Our findings demonstrated that flavonoids from Astragalus membranaceus ameliorated brain impairment, and its modulation on gut-brain axis plays a pivotal role. Our present study provided an alternative solution on preventing and treating diabetic cognition impairment.

【 授权许可】

Unknown