期刊论文详细信息
Microorganisms
Probiotic and Metabolic Characterization of Vaginal Lactobacilli for a Potential Use in Functional Foods
Lorenzo Siroli1  Rosalba Lanciotti1  Margherita D’Alessandro1  Francesca Patrignani1  Carola Parolin2  Beatrice Vitali2  Maria De Angelis3  Danka Bukvicki4 
[1] Department of Agricultural and Food Sciences, University of Bologna, 47521 Cesena, Italy;Department of Pharmacy and Biotechnology, University of Bologna, 40127 Bologna, Italy;Department of Soil, Plant, and Food Sciences, University of Bari Aldo Moro, 70126 Bari, Italy;Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, 11000 Belgrade, Serbia;
关键词: probiotic;    functional characterization;    Lactobacillus crispatus;    Lactobacillus gasseri;    Limosilactobacillus vaginalis;    biolog;   
DOI  :  10.3390/microorganisms9040833
来源: DOAJ
【 摘 要 】

The main aim of this work was to verify the metabolic and functional aptitude of 15 vaginal strains belonging to Lactobacillus crispatus, Lactobacillus gasseri, and Limosilactobacillus vaginalis (previously Lactobacillus vaginalis), already characterized for their technological and antimicrobial properties. In order to evaluate the metabolic profile of these vaginal strains, a phenotype microarray analysis was performed on them. Functional parameters such as hydrophobicity, auto-aggregation, deconjugation of bile salts, adhesion to an intestinal cell line (Caco-2), and a simulated digestion process were evaluated for these strains. A good number of these strains showed hydrophobicity values higher than 70%. Regarding the auto-aggregation assay, the most promising strains were L. crispatus BC9 and BC1, L. gasseri BC10 and BC14, and L. vaginalis BC17. Moreover, L. crispatus BC4, BC6, BC7, and BC8 were characterized by strong bile salts hydrolase activity (BHS). In addition, L. crispatus BC8 and L. vaginalis BC17 were characterized by a medium ability to adhere to Caco-2 cells. Data related to digestion process showed a strong ability of vaginal lactobacilli to withstand this stress. In conclusion, the data collected show the metabolic versatility and several exploitable functional properties of the investigated vaginal lactobacilli.

【 授权许可】

Unknown   

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