Oral Administration of Lactobacillus helveticus LA401 and Lactobacillus gasseri LA806 Combination Attenuates Oesophageal and Gastrointestinal Candidiasis and Consequent Gut Inflammation in Mice

Journal of Fungi

Sarah Kuylle¹  Sophie Holowacz²  Claude Blondeau²  Marie Salon³  Mouna Rahabi³  Agnès Coste³  Hélène Authier³  Bénédicte Bertrand³ 

[1] GENIBIO, 91290 Lorp-Sentaraille, France;PiLeJe Laboratoire, 75015 Paris, France;UMR 152 Pharma-Dev, Université de Toulouse, IRD, UPS, 31432 Toulouse, France;

关键词: gastrointestinal candidiasis;    gut inflammation;    macrophages;    Lactobacillus gasseri;    Lactobacillus helveticus;    probiotic;   

DOI  :  10.3390/jof7010057

来源: DOAJ

【 摘 要 】

Candida albicans is an opportunistic pathogen that causes mucosal gastrointestinal (GI) candidiasis tightly associated with gut inflammatory status. The emergence of drug resistance, the side effects of currently available antifungals and the high frequency of recurrent candidiasis indicate that new and improved therapeutics are needed. Probiotics have been suggested as a useful alternative for the management of candidiasis. We demonstrated that oral administration of Lactobacillus gasseri LA806 alone or combined with Lactobacillus helveticus LA401 in Candida albicans-infected mice decrease the Candida colonization of the oesophageal and GI tract, highlighting a protective role for these strains in C. albicans colonization. Interestingly, the probiotic combination significantly modulates the composition of gut microbiota towards a protective profile and consequently dampens inflammatory and oxidative status in the colon. Moreover, we showed that L. helveticus LA401 and/or L. gasseri LA806 orient macrophages towards a fungicidal phenotype characterized by a C-type lectin receptors signature composed of Dectin-1 and Mannose receptor. Our findings suggest that the use of the LA401 and LA806 combination might be a promising strategy to manage GI candidiasis and the inflammation it causes by inducing the intrinsic antifungal activities of macrophages. Thus, the probiotic combination is a good candidate for managing GI candidiasis by inducing fungicidal functions in macrophages while preserving the GI integrity by modulating the microbiota and inflammation.

【 授权许可】

Unknown