In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells

Scientific African

Richwell Mhlanga¹  Xolani Peter²  Moses K. Langat³  Nyeleti Vukea⁴  Vuyelwa Jacqueline Tembu⁵  Xavier Siwe-Noundou⁵  Rui W.M. Krause⁵  Douglas Kemboi Magozwi⁶  Jo-Anne de la Mare⁷ 

[1] Corresponding authors at: Department of Chemistry, Tshwane University of Technology, Pretoria 0001, South Africa.;Department of Biochemistry and Microbiology, Biomedical Biotechnology Research Unit, Rhodes University, South Africa;Department of Chemistry, Rhodes University, Grahamstown 6140, South Africa;Defense Peace Safety and Security, Center for Science Innovation and Research (CSIR), Pretoria 0001, South Africa;Department of Biochemistry and Microbiology, Biomedical Biotechnology Research Unit, Rhodes University, South Africa;Department of Chemistry, Tshwane University of Technology, Pretoria 0001, South Africa;Science Department, Royal Botanic Gardens Kew, Richmond TW9 3DS, UK;

关键词: Euphorbia grandicornis;    Chemical constituents;    Cytotoxic activities;    MCF-7;    HCC70;    MCF-12A;   

DOI  :  

来源: DOAJ

【 摘 要 】

Euphorbia grandicornis Blanc is widely utilized in traditional medicine for a variety of ailments including body pains associated with skin irritations, inflammation, and snake or scorpion bites. Compounds from E. grandicornis were characterized using spectroscopic techniques, NMR, IR, MS, and melting points and alongside the extracts were evaluated for in vitro anticancer activity against several cancer cell lines. The root extract afforded known, β-glutinol (1), β-amyrin (2), 24-methylenetirucalla-8-en-3β-ol (3), tirucalla-8,25-diene-3β,24R-diol (4), stigmasterol (5), sitosterol (6), and hexyl (E)-3-(4‑hydroxy-3-methoxyphenyl)-2-propenoate (7) based on their NMR spectroscopic data for the first report in E. grandicornis. The extracts and isolated compounds were evaluated for anticancer activities against hormone receptor-positive breast cancer (MCF-7), triple-negative breast cancer (HCC70), and non-tumorigenic mammary epithelial (MCF-12A) cell lines. The CH2Cl2 extract exhibited potent, cytotoxicity against MCF-7, HCC70, and MCF-12A cells. The aerial extract exhibited IC50 values of 1.03, 0.301, and 1.68 µg/mL, and root extract displayed IC50 values of 0.83, 0.83 and 3.98 µg/mL against MCF-7, HCC70, and MCF-12A cells, respectively. The root extract thus showed selectivity for the cancer cell lines over the non-cancerous control cell line (SI = 4.80). Hexyl (E)-3-(4‑hydroxy-3-methoxyphenyl)-2-propenoate (7) showed significant activity with IC50 values of 23.41, 29.45 and 27.01 µM against MCF-7, HCC70 and MCF-12A cells, respectively, suggesting non-specific cytotoxicity.

【 授权许可】

Unknown