Novel Mechanical Strain Characterization of Ventilated ex vivo Porcine and Murine Lung using Digital Image Correlation

Frontiers in Physiology

David D. Lo¹  Mehrzad Tartibi²  Mona Eskandari³  Samaneh Sattari⁴  Mohammad Maghsoudi-Ganjeh⁴  Crystal A. Mariano⁴ 

[1] BREATHE Center, School of Medicine, University of California, Riverside, Riverside, CA, United States;Delbeat LLC, San Francisco, CA, United States;Department of Bioengineering, Riverside, CA, United States;Department of Mechanical Engineering, University of California, Riverside, Riverside, CA, United States;Division of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, CA, United States;

关键词: biomechanics;    digital image correlation;    strain;    deformation;    pulmonary mechanics;    lung;   

DOI  :  10.3389/fphys.2020.600492

来源: DOAJ

【 摘 要 】

Respiratory illnesses, such as bronchitis, emphysema, asthma, and COVID-19, substantially remodel lung tissue, deteriorate function, and culminate in a compromised breathing ability. Yet, the structural mechanics of the lung is significantly understudied. Classical pressure-volume air or saline inflation studies of the lung have attempted to characterize the organ’s elasticity and compliance, measuring deviatory responses in diseased states; however, these investigations are exclusively limited to the bulk composite or global response of the entire lung and disregard local expansion and stretch phenomena within the lung lobes, overlooking potentially valuable physiological insights, as particularly related to mechanical ventilation. Here, we present a method to collect the first non-contact, full-field deformation measures of ex vivo porcine and murine lungs and interface with a pressure-volume ventilation system to investigate lung behavior in real time. We share preliminary observations of heterogeneous and anisotropic strain distributions of the parenchymal surface, associative pressure-volume-strain loading dependencies during continuous loading, and consider the influence of inflation rate and maximum volume. This study serves as a crucial basis for future works to comprehensively characterize the regional response of the lung across various species, link local strains to global lung mechanics, examine the effect of breathing frequencies and volumes, investigate deformation gradients and evolutionary behaviors during breathing, and contrast healthy and pathological states. Measurements collected in this framework ultimately aim to inform predictive computational models and enable the effective development of ventilators and early diagnostic strategies.

【 授权许可】

Unknown