Xylella fastidiosa subsp. pauca Strains Fb7 and 9a5c from Citrus Display Differential Behavior, Secretome, and Plant Virulence

International Journal of Molecular Sciences

AlineMaria da Silva¹  PauloMarques Pierry¹  PauloAdriano Zaini²  AbhayaM. Dandekar²  MônicaNeli Alves³  AlineGomes de Souza⁴  Rafael Nascimento⁴  JessicaBrito de Souza⁴  HebréiaOliveira Almeida-Souza⁴  LuizRicardo Goulart⁴ 

[1] Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000 São Paulo SP, Brazil;Department of Plant Sciences, College of Agriculture and Environmental Sciences, University of California, Davis, 1 Shields Ave, Davis, CA 95616, USA;Department of Technology, School of Agricultural and Veterinary Studies, São Paulo State University (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane, 14884-900 Jaboticabal SP, Brazil;Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas, Bloco 2E, Campus Umuarama, 38400-902 Uberlandia MG, Brazil;

关键词: lipase;    hemolysin-like protein;    peptidase;    phytopathogen;    pathogenicity;    secretome;   

DOI  :  10.3390/ijms21186769

来源: DOAJ

【 摘 要 】

Xylella fastidiosa colonizes the xylem of various cultivated and native plants worldwide. Citrus production in Brazil has been seriously affected, and major commercial varieties remain susceptible to Citrus Variegated Chlorosis (CVC). Collective cellular behaviors such as biofilm formation influence virulence and insect transmission of X. fastidiosa. The reference strain 9a5c produces a robust biofilm compared to Fb7 that remains mostly planktonic, and both were isolated from symptomatic citrus trees. This work deepens our understanding of these distinct behaviors at the molecular level, by comparing the cellular and secreted proteomes of these two CVC strains. Out of 1017 identified proteins, 128 showed differential abundance between the two strains. Different protein families were represented such as proteases, hemolysin-like proteins, and lipase/esterases, among others. Here we show that the lipase/esterase LesA is among the most abundant secreted proteins of CVC strains as well, and demonstrate its functionality by complementary activity assays. More severe symptoms were observed in Nicotiana tabacum inoculated with strain Fb7 compared to 9a5c. Our results support that systemic symptom development can be accelerated by strains that invest less in biofilm formation and more in plant colonization. This has potential application in modulating the bacterial-plant interaction and reducing disease severity.

【 授权许可】

Unknown