Radiation inactivation analysis of kidney microvillar peptidases

FEBS Letters

Kenny, A.John¹  Fulcher, Ian S.¹  Ingram, Jean¹ 

[1] MRC Membrane Peptidase Research Group, Department of Biochemistry, University of Leeds, Leeds LS2 9JT, England

关键词: Brush border membrane Membrane peptidase Microvillus Radiation inactivation analysis;   

DOI  :  10.1016/0014-5793(86)80921-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Five membrane peptidases were studied by radiation inactivation analysis of pig kidney microvillar membranes. One heterodimeric enzyme, γ-glutamyl transferase, presented a target size corresponding to the dimeric M _r. The other enzymes are known to be homodimers. Three of these, aminopeptidase A, aminopep-tidase N and dipeptidyl peptidase IV, gave results clearly indicating the monomer to be the target and, hence, in this group the association of the subunits was not essential for activity. The target size for endopep-tidase-24.11 was intermediate between those for monomer and dimer and its functional state was not resolved by the experiments.

【 授权许可】

Unknown