Apoptosis in human hepatoma HepG2 cells induced by the phenolics of Tetrastigma hemsleyanum leaves and their antitumor effects in H22 tumor-bearing mice

Journal of Functional Foods

Fang Chen¹  Qianru Hui²  Hongyan Li³  Ran Chen⁴  Zeyuan Deng⁵  Yong Sun⁵  Han Peng⁵ 

[1] Corresponding authors.;Guelph Food Research and Development Centre, Agricultural and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario N1G 5C9, Canada;CAS Key Laboratory of Systems Biology, Innovation Center for Cell Signaling Network, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China;State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China;

关键词: Antitumor effects;    Apoptosis;    LC-QTOF-MS/LC-QqQ-MS;    Mitochondria pathway;    Phenolics;    Tetrastigma hemsleyanum leaves;   

DOI  :  

来源: DOAJ

【 摘 要 】

Tetrastigma hemsleyanum leaves (THL), a popular anticancer functional food in China, are used as nutrient or dietary supplement. However, the anticancer effects and the underlying mechanism of THL are still unknown. Thus, the phenolic profiles and anticancer activities of THL purified extract were investigated by a combination of chemical assays, LC-QTOF-MS/LC-QqQ-MS techniques, cell cultures and tumor-bearing mice model. It has been indicated that the TPC/TFC (421.63 and 343.38 mg/g DW) of THL extract were obviously increased after SPE purification, and the main phenolic components were apigenin and luteolin glycosides. Besides, THL could induce apoptosis by both of extrinsic death receptor pathway and intrinsic mitochondria pathway in HepG2 cells as well as inhibit the tumor growth, regulate Bcl-2 family proteins, and active the caspase family proteins in H22 tumor-bearing mice. Summarily, the findings clearly demonstrated that THL could be a potential functional food for the treatment and prevention of liver cancer.

【 授权许可】

Unknown