Expanding xylose metabolism in yeast for plant cell wall conversion to biofuels

eLife

Vivian Yaci Yu¹  N Louise Glass²  Elizabeth A Znameroski³  Soo Rin Kim⁴  Annsea Park⁴  Julie M Liang⁴  Kulika Chomvong⁴  Yuping Lin⁴  Joanna Feehan⁴  Xin Li⁴  Raíssa Estrela⁵  Jamie HD Cate⁶  Yong-Su Jin⁶ 

[1] Impossible Foods, Inc, Redwood City, United States;School of Food Science and Biotechnology, Kyungpook National University, Daegu, Republic of Korea;Department of Chemistry, University of California, Berkeley, Berkeley, United States;Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States;Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States;Institute for Genomic Biology, University of Illinois, Urbana, United States;

关键词: xylose;    hemicellulose;    biofuel;    xylodextrin;    cofermentation;    xylosyl-xylitol;   

DOI  :  10.7554/eLife.05896

来源: DOAJ

【 摘 要 】

Sustainable biofuel production from renewable biomass will require the efficient and complete use of all abundant sugars in the plant cell wall. Using the cellulolytic fungus Neurospora crassa as a model, we identified a xylodextrin transport and consumption pathway required for its growth on hemicellulose. Reconstitution of this xylodextrin utilization pathway in Saccharomyces cerevisiae revealed that fungal xylose reductases act as xylodextrin reductases, producing xylosyl-xylitol oligomers as metabolic intermediates. These xylosyl-xylitol intermediates are generated by diverse fungi and bacteria, indicating that xylodextrin reduction is widespread in nature. Xylodextrins and xylosyl-xylitol oligomers are then hydrolyzed by two hydrolases to generate intracellular xylose and xylitol. Xylodextrin consumption using a xylodextrin transporter, xylodextrin reductases and tandem intracellular hydrolases in cofermentations with sucrose and glucose greatly expands the capacity of yeast to use plant cell wall-derived sugars and has the potential to increase the efficiency of both first-generation and next-generation biofuel production.

【 授权许可】

Unknown