Xylose‐specific antibodies as markers of subcompartmentation of terminal glycosylation in the Golgi apparatus of sycamore cells

FEBS Letters

Faye, Loïc¹  Gomord, Véronique¹  Lainé, Anne-Catherine¹ 

[1] Laboratoire des Transports Intracellulaires, CNRS-UA 203, Université de Rouen, Facultè des Sciences, F-76134 Mont Saint Aignan, France

关键词: Xylosyltransferase immunolocalization;    Plant glycoprotein biosynthesis;    Plant glycosyltransferase;    Plant Golgi apparatus;    GlcNAc;    N-acetylglucosamine;    Man;    mannose;    Xyl;    xylose;    Fuc;    L-fucose;    Gal;    galactose;    RER;    rough endoplasmic reticulum;    TGN;    trans Golgi network;   

DOI  :  10.1016/0014-5793(91)81413-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antibodies specific for xylose-containing plant complex N-linked glycans are used for indirect immunolocalization of xylosyltransferase in sycamore cells. The use of high pressure freezing and freeze substitution for sample preparation resulted in very good morphological preservation of the different Golgi cisternae. Xylosyltransferase shows a diffuse distribution all over the Golgi stacks and xylosylation appears to be an early processing event that is initiated in the cis Golgi compartment.

【 授权许可】

Unknown