Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System

Química Nova

Ana Lúcia Figueiredo Porto¹  Tatiana Souza Porto¹  Larissa de Souza Kirsch²  Maria Francisca Simas Teixeira²  Ana Carolina dos Santos Pinto² 

[1] Universidade Federal Rural de Pernambuco;Universidade Federal do Amazonas;

关键词: Lentinus citrinus;    proteases;    aqueous two-phase systems;   

DOI  :  10.1590/S0100-40422012001000004

来源: DOAJ

【 摘 要 】

A full two-level factorial design was employed to study the influence of PEG molar mass (MM PEG), PEG concentration (C PEG) and phosphate concentration (C PHOSPH) on proteases partition by Lentinus citrinus DPUA 1535 in a PEG/phosphate aqueous two-phase system (ATPS). For all ATPS studied, proteases partitioned for the top phase and the best proteases extraction condition was obtained with MM PEG = 6000 g mol-1, C PEG = 17.5% (w/w) and C PHOSPH = 25% (w/w) with (1.1) purification factor and (151%) activity yield. Findings reported here demonstrate a practical strategy that serves as a first step for proteases purification from crude extract by L. citrinus.

【 授权许可】

Unknown