Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System

Química Nova

Universidade Federal do Amazonas, Manaus, Brasil¹  Pinto, Ana Carolina dos Santos¹  Kirsch, Larissa de Souza¹  Universidade Federal Rural de Pernambuco, Garanhuns, Brasil¹  Universidade Federal Rural de Pernambuco,¹  Porto, Ana Lúcia Figueiredo¹  Porto, Tatiana Souza¹  Teixeira, Maria Francisca Simas¹ 

关键词: Lentinus citrinus;    proteases;    aqueous two-phase systems.;   

DOI  :  10.1590/S0100-40422012001000004

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A full two-level factorial design was employed to study the influence of PEG molar mass (MMPEG), PEG concentration (CPEG) and phosphate concentration (CPHOSPH) on proteases partition by Lentinus citrinus DPUA 1535 in a PEG/phosphate aqueous two-phase system (ATPS). For all ATPS studied, proteases partitioned for the top phase and the best proteases extraction condition was obtained with MMPEG = 6000 g mol-1, CPEG = 17.5% (w/w) and CPHOSPH = 25% (w/w) with (1.1) purification factor and (151%) activity yield. Findings reported here demonstrate a practical strategy that serves as a first step for proteases purification from crude extract by L. citrinus.

【 授权许可】

Unknown