Partition of proteases from Lentinus citrinus DPUA 1535 by the Peg/Phosphate Aqueous Two-Phase System

Quimica nova

Porto, Ana Lúcia Figueiredo¹  Porto, Tatiana Souza²  Pinto, Ana Carolina dos Santos³  Kirsch, Larissa de Souza³  Teixeira, Maria Francisca Simas³ 

[1] Universidade Federal Rural de Pernambuco,;Universidade Federal Rural de Pernambuco, Garanhuns, Brasil;Universidade Federal do Amazonas, Manaus, Brasil

关键词: Lentinus citrinus;    proteases;    aqueous two-phase systems. ;     ;    INTRODUCTION;   

DOI  :  10.1590/S0100-40422012001000004

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A full two-levelfactorial design was employed to study the influence of PEG molar mass (MMPEG),PEG concentration (CPEG) and phosphate concentration (CPHOSPH)on proteases partition by Lentinus citrinus DPUA 1535 in a PEG/phosphateaqueous two-phase system (ATPS). For all ATPS studied, proteases partitionedfor the top phase and the best proteases extraction condition was obtained withMMPEG = 6000 g mol-1, CPEG = 17.5% (w/w) andCPHOSPH = 25% (w/w) with (1.1) purification factor and (151%) activityyield. Findings reported here demonstrate a practical strategy that serves asa first step for proteases purification from crude extract by L. citrinus.

【 授权许可】

CC BY