Rapid Identification of Antioxidant Compounds ofGenista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS

Molecules

Sabrina Boutefnouchet¹  Sylvie Michel¹  Grégory Genta-Jouve¹  Djamila Meriane²  Mohamed Kaabeche² 

[1] Laboratoire de Pharmacognosie, U.M.R./C.N.R.S. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, 4 Avenue de l'Observatoire,F-75006 Paris, France;Laboratoire de Phytothérapie Appliquée aux Maladies Chroniques, Université Ferhat Abbas,Sétif 19000, Algeria;

关键词: antioxidant;    HPTLC-MS;    Genista saharae;    DPPH scavenging assay;   

DOI  :  10.3390/molecules19044369

来源: DOAJ

【 摘 要 】

Genista species are sources of antioxidant phenolic compounds such as O- and C-glycosylflavonoids and isoflavonoids. A combination of a DPPH scavenging assay with HPTLC-MS, a fast and efficient method for identification of bioactive compounds, has been applied for evaluation of the radical scavenging activity of metabolites from Genista saharae Coss. & Dur. Different organs collected at various periods have been compared. Identification of antioxidant compounds was obtained by elution of the major DPPH-inhibition zones. The resulting HPTLC-MS analysis under moderately polar conditions, coupled to the DPPH results led to the putative identification of two antioxidant isoflavoneaglycones: 3',4',5,7-tetrahydroxyisoflavone (1) and ficuisoflavone (3), whereas polar migration conditions led to the identification of the glycosides 5-methoxy-4',7-trihydroxy-8-glucopyranosylisoflavone (4) and 4',5-dihydroxy-7-methoxyisoflavone-4'-O-β-D-gluco-pyranoside (5). Evaluation of percentage of inhibition of DPPH radical by the purified isoflavone 4 from the root extract showed that it affords a moderate contribution to the total radical scavenging activity of the extract.

【 授权许可】

Unknown