In Vitro Cell Interactions on PVDF Films: Effects of Surface Morphology and Polar Phase Transition

Materials

Maria Giovanna Pastore Carbone¹  Vincenzo Guarino²  Valentina Cirillo²  Pellegrino Musto³  Marianna Pannico³  Marco A. Alvarez-Perez⁴ 

[1] Institute of Chemical Engineering Sciences, Foundation for Research, and Technology Hellas (FORTH-ICEHT), Stadiou St, Platani GR-26504, 26504 Patras, Greece;Institute of Polymers, Composites and Biomaterials (IPCB), National Research Council of Italy, Mostra d’Oltremare Pad.20, Viale J.F. Kennedy 54, 80125 Naples, Italy;Institute of Polymers, Composites and Biomaterials, National Research Council of Italy, Via Campi Flegrei 32, 80078 Pozzuoli, Italy;TBL-DEPeI, Universidad Nacional Autonoma de Mexico (UNAM), Mexico City 04510, Mexico;

关键词: morphology;    AFM;    in vitro response;    hMSC;    piezoelectric materials;   

DOI  :  10.3390/ma14185232

来源: DOAJ

【 摘 要 】

In recent years, several studies have validated the use of piezoelectric materials for in situ biological stimulation, opening new interesting insights for bio-electric therapies. In this work, we investigate the morphological properties of polyvinylidene fluoride (PVDF) in the form of microstructured films after temperature-driven phase transition. The work aims to investigate the correlations between morphology at micrometric (i.e., spherulite size) and sub-micrometric (i.e., phase crystallinity) scale and in vitro cell response to validate their use as bio-functional interfaces for cellular studies. Morphological analyses (SEM, AFM) enabled evidence of the peculiar spherulite-like structure and the dependence of surface properties (i.e., intra-/interdomain roughness) upon process conditions (i.e., temperature). Meanwhile, chemical (i.e., FTIR) and thermal (i.e., DSC) analyses highlighted an influence of casting temperature and polymer solution on apolar to polar phases transition, thus affecting in vitro cell response. Accordingly, in vitro tests confirmed the relationship between micro/sub-microstructural properties and hMSC response in terms of adhesion and viability, thus suggesting a promising use of PVDF films to model, in perspective, in vitro functionalities of cells under electrical stimuli upon mechanical solicitation.

【 授权许可】

Unknown