Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance

Frontiers in Cell and Developmental Biology

Silvia Taccola¹  Matthew A. A. Smith¹  Robert W. Kay¹  Russell A. Harris¹  Steven D. R. Christie²  Maria Pardo-Figuerez³  Andrew J. Capel³  Rowan P. Rimington³  Mark P. Lewis³ 

[1] Faculty of Engineering and Physical Sciences, School of Mechanical Engineering, University of Leeds, Leeds, United Kingdom;School of Science, Loughborough University, Loughborough, United Kingdom;School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, United Kingdom;

关键词: direct write;    aerosol jet printing;    microfabrication;    neuronal alignment;    tissue engineering;    biomaterials;   

DOI  :  10.3389/fcell.2021.722294

来源: DOAJ

【 摘 要 】

Digitally driven manufacturing technologies such as aerosol jet printing (AJP) can make a significant contribution to enabling new capabilities in the field of tissue engineering disease modeling and drug screening. AJP is an emerging non-contact and mask-less printing process which has distinct advantages over other patterning technologies as it offers versatile, high-resolution, direct-write deposition of a variety of materials on planar and non-planar surfaces. This research demonstrates the ability of AJP to print digitally controlled patterns that influence neuronal guidance. These consist of patterned poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) tracks on both glass and poly(potassium 3-sulfopropyl methacrylate) (PKSPMA) coated glass surfaces, promoting selective adhesion of SH-SY5Y neuroblastoma cells. The cell attractive patterns had a maximum height ≥0.2 μm, width and half height ≥15 μm, Ra = 3.5 nm, and RMS = 4.1. The developed biocompatible PEDOT:PSS ink was shown to promote adhesion, growth and differentiation of SH-SY5Y neuronal cells. SH-SY5Y cells cultured directly onto these features exhibited increased nuclei and neuronal alignment on both substrates. In addition, the cell adhesion to the substrate was selective when cultured onto the PKSPMA surfaces resulting in a highly organized neural pattern. This demonstrated the ability to rapidly and flexibly realize intricate and accurate cell patterns by a computer controlled process.

【 授权许可】

Unknown