Optimization of antigen-specific CD8+ T cell activation conditions for infectious diseases including COVID-19

STAR Protocols

Lijuan Gao¹  Jiezhou Ye²  Guobing Chen²  Congling Qiu²  Oscar Junhong Luo²  Chanchan Xiao³  Jieping Deng³  Jun Su³  Pengcheng Wang³ 

[1] Affiliated Huaqiao Hospital, Jinan University, Guangzhou, China;Guangdong-Hong Kong-Macau Great Bay Area Geroscience Joint Laboratory, Guangzhou, China;Department of Microbiology and Immunology, Institute of Geriatric Immunology, School of Medicine, Jinan University, Guangzhou, China;

关键词: Cell Biology;    Cell isolation;    Immunology;    Microbiology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Here, we describe the use of the artificial antigen-presenting cell (aAPC) system for the verification of T-cell epitopes. We purify and activate CD8+ T cells from blood samples from HLA-A2 that are negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). CD8+ T cells are combined with peptide-loaded T2-A2 cells, which are then stained with a SARS-CoV-2-specific MHC-1 tetramer to identify specific HLA-A2-restricted T-cell epitopes. The use of aAPC and healthy donors means that only BSL2 lab conditions are needed.For details of the use and implementation of this protocol, please refer to Deng et al. (2021).

【 授权许可】

Unknown