Data in support of protocol for rat single muscle-fiber isolation and culture

Data in Brief

Judy E. Anderson¹  Yusuke Komiya²  Mariko Akahoshi²  Yoshihide Ikeuchi²  Mako Nakamura²  Ryuichi Tatsumi²  Wataru Mizunoya² 

[1] Department of Biological Sciences, University of Manitoba, Winnipeg, Canada R3T 2N2;Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan;

关键词: Skeletal muscle;    Muscle fiber type;    Primary culture;    Myotube;    Western blotting;   

DOI  :  10.1016/j.dib.2015.04.016

来源: DOAJ

【 摘 要 】

This data article contains data related to the research article entitled, "Protocol for rat single muscle-fiber isolation and culture" by Komiya et al. [1]. It has yet to be shown whether adult myosin heavy chain (MyHC) isoforms are expressed at a readily detectable level in cultured myotubes. In this study, we examined whether adult MyHC isoforms could be detected in myotubes differentiated from rat satellite cells using a Western blotting assay and specific antibodies against slow MyHC, fast MyHC and pan-MyHC. Results showed that slow adult MyHC isoforms were faintly detected in myotubes, suggesting that rat myotubes express adult MyHC isoforms although that amount is very low.

【 授权许可】

Unknown