Comparison between Exhaustive and Equilibrium Extraction Using Different SPE Sorbents and Sol-Gel Carbowax 20M Coated FPSE Media

Molecules

Halil I. Ulusoy¹  Kenneth G. Furton²  Abuzar Kabir²  Silvia Piccolantonio³  Angela Tartaglia³  Fausto Croce³  Marcello Locatelli³  Fabio Maroni³  Pantaleone Bruni³  Daniela Macerola³  Elena Sperandio³  Victoria F. Samanidou⁴ 

[1] Department of Analytical Chemistry, Faculty of Pharmacy, Cumhuriyet University, Sivas 58140, Turkey;Department of Chemistry and Biochemistry, International Forensic Research Institute, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA;Department of Pharmacy, University of Chieti–Pescara “G. d’Annunzio”, Via dei Vestini 31, 66100 Chieti, Italy;Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece;

关键词: FPSE;    in-house loaded SPE;    HPLC-PDA;    method validation;    IBD;    extraction;   

DOI  :  10.3390/molecules24030382

来源: DOAJ

【 摘 要 】

This paper reports the performance comparison between the exhaustive and equilibrium extraction using classical Avantor C18 solid phase extraction (SPE) sorbent, hydrophilic-lipophilic balance (HLB) SPE sorbent, Sep-Pak C18 SPE sorbent, novel sol-gel Carbowax 20M (sol-gel CW 20M) SPE sorbent, and sol-gel CW 20M coated fabric phase sorptive extraction (FPSE) media for the simultaneous extraction and analysis of three inflammatory bowel disease (IBD) drugs that possess logP values (polarity) ranging from 1.66 for cortisone, 2.30 for ciprofloxacin, and 2.92 for sulfasalazine. Both the commercial SPE phases and in-house synthesized sol-gel CW 20M SPE phases were loaded in SPE cartridges and the extractions were carried out under an exhaustive extraction mode. FPSE was carried out under an equilibrium extraction mode. The drug compounds were resolved using a Luna C18 column (250 mm × 4.6 mm; 5 m particle size) in gradient elution mode within 20 min and the method was validated in compliance with international guidelines for the bioanalytical method validation. Novel in-house synthesized and loaded sol-gel CW 20M SPE sorbent cartridges were characterized in terms of their extraction capability, breakthrough volume, retention volume, hold-up volume, number of the theoretical plate, and the retention factor.

【 授权许可】

Unknown