Anti-proliferative bioactivity against HT-29 colon cancer cells of a withanolides-rich extract from golden berry (Physalis peruviana L.) calyx investigated by Foodomics

Journal of Functional Foods

Gerardo Alvarez-Rivera¹  Sandra Johanna Morantes²  Alejandro Cifuentes³  Alberto Valdés⁴  Diego Ballesteros-Vivas⁴  Carlos León⁴  Fabián Parada-Alfonso⁴  Elena Ibánez⁵ 

[1] High Pressure Laboratory, Department of Chemistry, Faculty of Science, Universidad Nacional de Colombia, Carrera 30 45-03, Bogotá D.C. 111321, Colombia;Department of Bioengineering, Universidad Carlos III de Madrid, Leganés, Madrid, Spain;High Pressure Laboratory, Department of Chemistry, Faculty of Science, Universidad Nacional de Colombia, Carrera 30 45-03, Bogotá D.C. 111321, Colombia;Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC, Nicolás Cabrera 9, 28049 Madrid, Spain;Unit of Basic Oral Investigation (UIBO), Universidad El Bosque, Av. Carrera 9 131 A-02, Bogotá D.C. 110121, Colombia;

关键词: Goldenberry calyx;    Colon cancer;    Anti-proliferative activity;    Metabolomics;    Transcriptomics;    Foodomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

A comprehensive Foodomics study was carried out in this work to investigate the changes induced at gene and metabolite expression levels on HT29 colon cancer cell lines upon treatment with a bioactives-enriched extract from goldenberry calyx. As a result of the proposed multi-omics approach, the tested extract induced transcriptional activation of pro-apoptotic genes, and altered the expression of several genes related to the oxidative stress response in treated cancer cells. Metabolomics data confirm altered cellular redox homeostasis, providing additional evidence to transcriptomic results. Foodomics data integration also revealed alteration on relevant metabolic processes, suggesting inactivation of aminoacyl tRNA charging pathway, dysfunction on carnitine shuttle and beta-oxidation of fatty acids, and pyrimidine ribonucleotide interconversion impairment. These observations are in line with functional analysis and anti-proliferative activity results, where the viability of HT-29 colon cancer cells was notably reduced after 48 h treatment without affecting the viability of normal human colon fibroblast cells.

【 授权许可】

Unknown