Cooperative processing of primary miRNAs by DUS16 and DCL3 in the unicellular green alga Chlamydomonas reinhardtii

Communicative & Integrative Biology

Tomohito Yamasaki¹  Heriberto Cerutti² 

[1] National Institute for Basic Biology;School of Biological Science and Center for Plant Science Innovation, University of Nebraska–Lincoln;

关键词: Argonaute;    Chlamydomonas reinhardtii;    Dicer;    miRNA;    RNA-binding protein;    small RNA-seq;   

DOI  :  10.1080/19420889.2017.1280208

来源: DOAJ

【 摘 要 】

We have previously reported that the RNA-binding protein Dull slicer 16 (DUS16) plays a key role in the processing of primary miRNAs (pri-miRNAs) in the unicellular green alga Chlamydomonas reinhardtii. In the present report, we elaborate on the interaction of DUS16 with Dicer-like 3 (DCL3) during pri-miRNA processing. Comprehensive analyses of small RNA libraries derived from mutant and wild-type algal strains allowed the de novo prediction of 35 pri-miRNA genes, including 9 previously unknown ones. The pri-miRNAs dependent on DUS16 for processing largely overlapped with those dependent on DCL3. Our findings suggest that DUS16 and DCL3 work cooperatively, presumably as components of a microprocessor complex, in the processing of the majority of pri-miRNAs in C. reinhardtii.

【 授权许可】

Unknown