Mycobacterium avium subsp. paratuberculosis Proteome Changes Profoundly in Milk

Metabolites

Ricarda Mayer¹  Erwin Märtlbauer¹  Christina Hölzel¹  Cornelia A. Deeg²  Bernhard F. Hobmaier²  Roxane L. Degroote²  Kristina J. H. Kleinwort²  Stefanie M. Hauck³ 

[1] Chair of Hygiene and Technology of Milk, Department of Veterinary Sciences, LMU Munich, D-85764 Oberschleißheim, Germany;Chair of Physiology, Department of Veterinary Sciences, LMU Munich, D-82152 Martinsried, Germany;Research Unit Protein Science, Helmholtz Center Munich, German Research Center for Environmental Health, D-80939 Munich, Germany;

关键词: Mycobacterium avium subspecies paratuberculosis;    dairy product;    milk;    differential protein expression;    food safety;    LC–MS/MS;   

DOI  :  10.3390/metabo11080549

来源: DOAJ

【 摘 要 】

Mycobacterium avium subspecies paratuberculosis (MAP) are detectable viable in milk and other dairy products. The molecular mechanisms allowing the adaptation of MAP in these products are still poorly understood. To obtain information about respective adaptation of MAP in milk, we differentially analyzed the proteomes of MAP cultivated for 48 h in either milk at 37 °C or 4 °C or Middlebrook 7H9 broth as a control. From a total of 2197 MAP proteins identified, 242 proteins were at least fivefold higher in abundance in milk. MAP responded to the nutritional shortage in milk with upregulation of 32% of proteins with function in metabolism and 17% in fatty acid metabolism/synthesis. Additionally, MAP upregulated clusters of 19% proteins with roles in stress responses and immune evasion, 19% in transcription/translation, and 13% in bacterial cell wall synthesis. Dut, MmpL4_1, and RecA were only detected in MAP incubated in milk, pointing to very important roles of these proteins for MAP coping with a stressful environment. Dut is essential and plays an exclusive role for growth, MmpL4_1 for virulence through secretion of specific lipids, and RecA for SOS response of mycobacteria. Further, 35 candidates with stable expression in all conditions were detected, which could serve as targets for detection. Data are available via ProteomeXchange with identifier PXD027444.

【 授权许可】

Unknown