Isolation of nuclei from mouse white adipose tissues for single-nucleus genomics

STAR Protocols

Ellen Gammelmark¹  Elvira Laila Van Hauwaert²  Jesper Grud Skat Madsen²  Anitta Kinga Sárvári²  Ronni Nielsen²  Lena Larsen²  Susanne Mandrup² 

[1] Corresponding author;Center for Functional Genomics and Tissue Plasticity, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M 5230, Denmark;

关键词: Cell isolation;    Single Cell;    Genomics;    RNA-seq;    Metabolism;    Gene Expression;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Lipid-filled adipocytes are incompatible with droplet-based single-cell methods, such as 10x Genomics-based technology, thus restricting droplet-based single-cell analyses of adipose tissues to the stromal vascular fraction. To overcome this limitation and obtain cellular and molecular insight into adipose tissue composition and plasticity, single-nucleus sequencing-based technologies can be applied. Here, we provide an optimized protocol for nuclei isolation from mouse adipose tissues suitable for single-nucleus RNA sequencing. This allows for transcriptomic profiling of the entire adipose tissue at single-cell resolution.For complete details on the use of this protocol, please refer to Sárvári et al., 2021.

【 授权许可】

Unknown