Size-Dependent Internalization Efficiency of Macrophages from Adsorbed Nanoparticle-Based Monolayers

Nanomaterials

Karine Anselme¹  Laurent Pieuchot¹  Tatiana Petithory¹  Arnaud Ponche¹  Laurent Vonna¹  Ludovic Josien¹ 

[1] Institut de Science des Matériaux de Mulhouse, Université de Haute-Alsace, 68057 Mulhouse, France;

关键词: nanoparticles;    monolayers;    physisorption;    macrophages;    internalization;    endocytosis;   

DOI  :  10.3390/nano11081963

来源: DOAJ

【 摘 要 】

Functional coatings based on the assembly of submicrometric or nanoparticles are found in many applications in the biomedical field. However, these nanoparticle-based coatings are particularly fragile since they could be exposed to cells that are able to internalize nanoparticles. Here, we studied the efficiency of RAW 264.7 murine macrophages to internalize physisorbed silica nanoparticles as a function of time and particle size. This cell internalization efficiency was evaluated from the damages induced by the cells in the nanoparticle-based monolayer on the basis of scanning electron microscopy and confocal laser scanning microscopy observations. The internalization efficiency in terms of the percentage of nanoparticles cleared from the substrate is characterized by two size-dependent regimes. Additionally, we highlighted that a delay before internalization occurs, which increases with decreasing adsorbed nanoparticle size. This internalization is characterized by a minimal threshold that corresponds to 35 nm nanoparticles that are not internalized during the 12-h incubation considered in this work.

【 授权许可】

Unknown