Apigenin modulates hippocampal CREB-BDNF signaling in high fat, high fructose diet-fed rats

Journal of Functional Foods

Kalpana Kalaivanan¹  Sathiya Priya Chandrasekaran²  Vidhya Ramachandran²  Anuradha Carani Venkatraman²  Jagan Kalivarathan²  Dipti Nanda² 

[1] Division of Transplantation, Department of Surgery, Virginia Commonwealth University, Richmond, VA, USA;Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India;

关键词: High fat, high fructose diet;    Dipeptidyl peptidase-4;    Brain-derived neurotrophic factor;    Insulin resistance;    Apigenin;    Sitagliptin;   

DOI  :  

来源: DOAJ

【 摘 要 】

A high-calorie diet is associated with brain insulin resistance and neuronal dysfunction. Brain-derived neurotrophic factor (BDNF) plays a role in neurogenesis, cognitive functions and synaptic plasticity. Insulin resistance significantly alters BDNF levels which makes neurons vulnerable to degeneration. Glucagon-like polypeptide (GLP)-1, an insulin secretagogue is shown to influence BDNF signaling through cAMP response element-binding protein (CREB). Apigenin (API), a naturally occurring flavone has potent antioxidant and anti-inflammatory properties. Although the neuroprotective effect of API has been shown in some studies, the effect of API on brain insulin resistance and on BDNF-CREB signaling still remains elusive. The aim of this study is to evaluate the effect of API on behavioral changes, insulin signaling and CREB-BDNF axis in hippocampus of high fat, high fructose diet (HFFD)-fed rats. The underlying mechanisms were elucidated using sitagliptin (STG), an inhibitor of dipeptidyl peptidase (DPP)-4 as a standard drug for comparison. Our results show that API has significant inhibitory potential against DPP-4 comparable to STG. Administration of API significantly improved GLP-1 levels, cognitive function, insulin signaling and influenced the CREB-BDNF axis. Chromatin immunoprecipitation (ChIP) assay revealed that the binding of CREB protein to the promoter IV of the BDNF gene is increased in API treated animals. Our findings suggest that API could modulate brain insulin signaling during calorie excess by upregulating BDNF signaling through its ability to enhance GLP-1.

【 授权许可】

Unknown