期刊论文详细信息
Journal of Lipid Research | |
Release of carbohydrates from sphingoglycolipid by osmium-catalyzed periodate oxidation followed by treatment with mild alkali | |
Sen-Itiroh Hakomori1  | |
[1] Laboratory for Carbohydrate Research, Departments of Biological Chemistry and Medicine, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts; | |
关键词: osmium-catalyzed; periodate; oxidation; acetylated; sphingoglycolipid; cleavage; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
The carbohydrate moiety of sphingoglycolipid, after preliminary acetylation, can be released by periodate oxidation catalyzed by a trace amount of osmium tetroxide, followed by alkaline treatment.Cerebroside, lactosyl ceramide, hematoside, globoside, and gangliosides were degraded to yield, respectively, galactose, lactose, sialyl lactose, a tetrasaccharide, and various oligosaccharides containing sialic acid. Oligosaccharides were separated by paper chromatography and paper electrophoresis. The procedure is useful for characterizing micromolar amounts of sphingoglycolipids.
【 授权许可】
Unknown