Identification of POMC processing products in single melanotrope cells by matrix‐assisted laser desorption/ionization mass spectrometry

FEBS Letters

Jespersen, S.¹  Roubos, E.W.²  van der Greef, J.¹  Jenks, B.G.²  van Striena, F.J.C.² 

[1] Netherlands Organization for Applied Scientific Research (TNO), Centre for Structure Elucidation and Instrumental Analysis, P. O. Box 360, 3 700 AJ Zeist, The Netherlands;Department of Animal Physiology, Nijmengen Institute for Neurosciences, Subfaculty of Biology, University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands

关键词: Matrix-assisted laser desorption/ionization mass spectrometr;    Proopiomelanocortin;    Melanotrope cell;    Pituitary;    Xenopus laevis;    Ac;    acetylated;    AJP;    acidic joining peptide;    ACTH;    adrenocorticotropic hormone;    CLIP;    corticotropin-like intermediate peptide;    DHB;    2;    5-dihydroxybenzoic acid;    LPH;    lipotropin;    MALDIMS;    matrix-assisted laser desorption/ionization mass spectrometry;    MSH;    melanocyte-stimulating hormone;    POW;    proopiomelanocortin;    TOF;    time-of-flight;   

DOI  :  10.1016/0014-5793(95)01503-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The use of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in identifying proopiomelanocortin (POMC) processing products in melanotrope cells of the pituitary intermediate lobe of Xenopus laevis was explored. Mass spectra were obtained with such a high sensitivity of detection that the peptides could be identified in a single melanotrope cell. In addition to known POMC processing products of the Xenopus melanotrope cell, t he presence of previously unidentified POMC-derived peptides was demonstrated. Together these POMC processing products accounted for the entire length of the POMC precursor. Furthermore, Xenopus possesses two genes for POMC and the sensitivity and accuracy of the MALDI-MS technique allowed identification of processing products of both the POMC_A and POMC_B gene. In addition, differences were obtained between the mass spectra of melanotrope cells from Xenopus laevis adapted to different conditions of background illumination. These results show that MALDI-MS is a valuable tool in the study of the expression of peptides in single (neuroendocrine) cells.

【 授权许可】

Unknown