One-pot production of butyl butyrate from glucose using a cognate “diamond-shaped” E. coli consortium

Bioresources and Bioprocessing

Guoxia Liu¹  Chunhua Zhao¹  Yanping Zhang¹  Yin Li¹  Jean Paul Sinumvayo¹ 

[1]CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences

关键词: Butyl butyrate;    E. coli;    Cognate consortium;    Butanol;    Butyrate;   

DOI  :  10.1186/s40643-021-00372-8

来源: DOAJ

【 摘 要 】

Abstract Esters are widely used in plastics, textile fibers, and general petrochemicals. Usually, esters are produced via chemical synthesis or enzymatic processes from the corresponding alcohols and acids. However, the fermentative production of esters from alcohols and/or acids has recently also become feasible. Here we report a cognate microbial consortium capable of producing butyl butyrate. This microbial consortium consists of two engineered butyrate- and butanol-producing E. coli strains with nearly identical genetic background. The pathways for the synthesis of butyrate and butanol from butyryl-CoA in the respective E. coli strains, together with a lipase-catalyzed esterification reaction, created a “diamond-shaped” consortium. The concentration of butyrate and butanol in the fermentation vessel could be altered by adjusting the inoculation ratios of each E. coli strain in the consortium. After optimization, the consortium produced 7.2 g/L butyl butyrate with a yield of 0.12 g/g glucose without the exogenous addition of butanol or butyrate. To our best knowledge, this is the highest titer and yield of butyl butyrate produced by E. coli reported to date. This study thus provides a new way for the biotechnological production of esters.

【 授权许可】

Unknown