Green Curmin Reduces Pro-inflammatory Cytokines and Fibroblast-Associated Colon Cancer Migration

Pharmaceutical Sciences

Tharathip Muangthong¹  Parichaya Aimvijarn¹  Prasit Suwannalert¹  Nasapon Povichit² 

[1] Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand.;Detox (Thailand) Co, Ltd., Chiangmai, Thailand.;

关键词: green curmin;    inflammation;    colon cancer;    fibroblast;    migration;   

DOI  :  10.34172/PS.2021.9

来源: DOAJ

【 摘 要 】

Background: Green Curmin is a soluble curcumin extract product made in Thailand that has been reported to reduce gastric inflammation. Inflammatory cytokines including IL-1α, IL-6,and TNF-β play a crucial role in cancer cell migration, which is a major development towards poor prognosis. The aim of this study is to investigate the effects of Green Curmin on colon cancer cell growth and migration, which involve pro-inflammatory cytokines. Methods: Curcuma longa L. were extracted and named Green Curmin. The cytotoxicity of theGreen Curmin-treated HCT116-colon cancer cells was obtained by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. The effect of the extract on cancer migration was investigated with a wound-healing assay. In addition, fibroblast-associated cancer migration was confirmed by using the Transwell migration assay. Proinflammatory cytokines were also determined. Results: Green Curmin had cytotoxicity in the induced-cancer cell apoptosis at concentrationsof 1 and 5 mg/ml. However, reduced colon cancer migration and fibroblast associated-cancermigration was found at concentrations of 0.05 and 0.1 mg/ml (non-toxicity dose), respectively. They also suppressed pro-inflammatory cytokines expression including IL-1α, IL-6, and TNF-βin colon cancer cells and IL-6 in fibroblast cells. Conclusion: Green Curmin has the potential to suppress pro-inflammatory cytokines and reduce fibroblast associated-cancer migration.

【 授权许可】

Unknown