A Method to Determine the Efficacy of a Commercial Phage Preparation against Uropathogens in Urine and Artificial Urine Determined by Isothermal Microcalorimetry

Microorganisms

Gernot Bonkat¹  Aurelia Pahnita Sigg²  Tecla Lafranca²  Anabel E. Grütter²  Max Mariotti²  Olivier Braissant²  Lorenz Leitner³ 

[1] Alta uro AG, Centralbahnplatz 6, 4051 Basel, Switzerland;Department of Biomedical Engineering, University of Basel, Gewerbestrasse 14, 4123 Allschwil, Switzerland;Department of Neuro-Urology, Balgrist University Hospital, University of Zürich, 8008 Zürich, Switzerland;

关键词: isothermal microcalorimetry;    bacteriophages;    phage cocktail;    E. coli;    P. mirabilis;   

DOI  :  10.3390/microorganisms10050845

来源: DOAJ

【 摘 要 】

Background: Urinary tract infections are commonly encountered and often treated with antibiotics. However, the inappropriate use of the latter has led to the appearance of resistant strains. In this context we investigate the use of calorimetry to rapidly determine if a phage cocktail can be used as alternative to antibiotics. Methods: We used a commercially available phage cocktail from an online pharmacy and tested it against a strain of Escherichia coli and a strain of Proteus mirabilis. We used isothermal microcalorimetry to follow the metabolic activity of the bacterial culture treated with the phage cocktail. Results: Isothermal microcalorimetry was able to follow the dynamic of the bacterial metabolic activity reduction by the phage cocktail. Both pathogens were strongly inhibited; however, some regrowth was observed for E. coli in urine. Conclusions: Isothermal microcalorimetry proved to be a valuable technique when investigating the efficacy of phage cocktails against uropathogens. We foresee that isothermal microcalorimetry could be used to obtain rapid phagograms.

【 授权许可】

Unknown